Designing primers potentially specific to Entamoeba gingivalis genes

Designing PCR Primers Painlessly†

Entamoeba gingivalis in Acute Osteomyelitis of the Mandible

An 86-year-old woman presented with osteonecrosis of the mandible following bisphosphonate therapy for multiple myeloma, and underwent surgical debridement and multiple dental extractions. Histopathologic examination of the necrotic bone fragments revealed acute osteomyelitis with mixed flora and organisms morphologically consistent with Entamoeba gingivalis. In addition to oral scrapings and s...

Frequency of Entamoeba gingivalis among periodontal and patients under chemotherapy.

This study was carried out to investigate the frequency of E. gingivalis in the gingival crevices of patients with periodontitis compared to normal subjects. Flagyl therapy was tried in cases with periodontitis. Also the frequency of this amoeba was investigated in patients receiving chemotherapy to be compared to normal subjects. The results revealed that higher frequency was observed in patie...

Detection of the amoeba Entamoeba gingivalis in periodontal pockets

Periodontitis is a public health issue, being one of the most prevalent diseases worldwide. However, the aetiology of the disease is still unclear: genetics of patients cannot explain the dispersed or isolated localisation of gingival pockets, while bacteria-based models are insufficient to distinguish gingivitis and periodontitis. The possible role of parasites in the establishment of periodon...

MethPrimer: designing primers for methylation PCRs

MOTIVATION DNA methylation is an epigenetic mechanism of gene regulation. Bisulfite- conversion-based PCR methods, such as bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP), remain the most commonly used techniques for methylation mapping. Existing primer design programs developed for standard PCR cannot handle primer design for bisulfite-conversion-based PCRs due to changes in ...